unc animal histopathology core

The UNC Microscopy Services Laboratory, Lineberger Comprehensive Cancer Center Animal Histopathology and Animal Studies Cores are all supported in part by an NCI Center Core Support Grant (CA016086) to the UNC Lineberger Comprehensive Cancer Center. Histopathology was blindly confirmed by a small-animal veterinarian specializing in gastrointestinal histopathology. Samples were analyzed at the UNC Flow Cytometry Core Facility on a CyAn and Summit 5.3 software was used to analyze data (Beckmann-Coulter, Indianapolis, IN). UNC Animal Histopathology Core. Non-canonical CH methylation mediated by DNMT3A during neuronal maturation has been recently shown to be critical for brain homeostasis. the Cystic Fibrosis Foundation grant BOUCHE19R0), and the UNC Lineberger Animal Histopathology Core for processing the cell culture membranes for paraffin embedding and H&E staining. The frozen serum is completely thawed before testing for ALT, AST, and BUN using standard enzymatic procedures by the UNC Animal Clinical Chemistry Core facility. Address correspondance to: Fei Sun, Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. ... Cellular homeostasis and adaptive mechanisms, histopathology, toxins/toxicants, infectious disease, senescence, and target tissues. 2012; Elmore et al. T cell–treated mice with tumor engrafted in kidney or testis were sacrificed and tumors were fixed in 10% neutral buffered formalin (Fisher Scientific), processed in 3-μm longitudinal planes, and stained for hematoxylin/eosin (H&E) and a human CD3 antibody (UNC Lineberger Animal Histopathology Core … Lisa E. Gralinski, Timothy P. Sheahan, Thomas E. Morrison, Vineet D. Menachery, Kara Jensen, Sarah R. Leist, Alan Whitmore, Mark T. Heise, Ralph S. Baric Animal Histopathology Core Facility. Tumor tissues were fixed in 10% paraformaldehyde (PFA), dehydrated, subsequently paraffin embedded, and sectioned within the LCCC Animal Histopathology Core Facility at the University of North Carolina at Chapel Hill. Cores with an asterisk (*) are live on CoreResearch@Duke . At least 20 µL is needed: 10 µL/test plus 10 µl for the added "dead volume" during a run. Inflammation is increasingly recognized as an important contributor to a host of CNS disorders; however, its regulation in the brain is not well delineated. The UNC Flow Cytometry Core Facility, the UNC Translational Pathology Laboratory, the Animal Histopathology and Laboratory Medicine Core, and the UNC Lineberger Animal Studies Core are supported in part by P30 CA016086 Cancer Center Core Support Grant to the UNC Lineberger Comprehensive Cancer Center. Two 5-micron thick Hematoxylin and Eosin stained lung sections (separated by 100 microns) are prepared and scored for a variety of metrics relating to the extent and severity of immune cell infiltration and pathological damage [slides were prepared and blind scored by the UNC Linberger Comprehensive Cancer Center Histopathology Core]. The Animal Histopathology and Lab Medicine Core’s clinical chemistry component assists research investigators who would greatly benefit from availability of service (on a cost-per-test basis) that replicates for animals the laboratory tests that are routine for humans. of the B2 bradykinin receptor-deficient animals have been done at the UNC site. • UNC established Microarray facility equipped in medium size printer and scanner. Mice and in vivo infection. Animal histopathology was performed within the LCCC Animal Histopathology Core Facility at the University of North Carolina at Chapel Hill, which is supported in part by NCI Center Core Support Grant (CA16086) to the UNC Lineberger Comprehensive Cancer Center. LCCC Animal Histopathology Core (919) 966-3653 lcccanimalistopathology@med.unc.edu 333 S. Columbia, 426 MacNider Hall University of North Carolina Chapel Hill, NC 27599. This core provides UNC investigators and other researchers with a full range of seamless services for animal pathology needs, from tissues to biologic fluids. Affiliations. Correctly targeted clones were transiently transfected with 20 µg of a CMV promoter–Cre expression, developed in the UNC Animal Models Core Facility, to remove the Neo cassette, and the result confirmed by PCR and Southern blot analysis. Failure of miR-29 to regulate the expression of Dnmt3a leads to severe neurobehavioral consequences. The Gnotobiotic Animal Core, which maintains germ-free and gnotobiotic rodents, is housed in the Laboratory Animal Resources facility at the College of Veterinary Medicine. Mice were euthanized via an overdose of ketamine 48 hours after the intravenous administration of different BEZ235 nanoformulations. As we continue to battle the COVID-19 pandemic, we must confront the possibility of new pathogenic coronaviruses emerging in humans in the future. Pitt Public Health's students and researchers explore disciplines to … The Atlas of Human Histology: A Guide to Microscopic Structure of Cells, Tissues and Organs by Robert L. Sorenson and T. Clark Brelje provides a print version of the core slides from this website. Although numerous epigenetic aberrancies accumulate in melanoma, their contribution to initiation and progression remain unclear. Animal Histopathology Core Nanoparticles (NPs). Animal histopathology was performed in the LCCC Animal Histopathology Core Facility at the University of North Carolina at Chapel Hill with special assistance from Traci Raley and Amanda Brown. The Gregory Lab and the Molecular Genomics Core The Blikslager Lab and the Gonzalez Lab at NC State and UNC’s Center for GI Biology and Disease ( CGIBD ) Large Animal Core Dr. Shannon McCall and Duke’s BioRepository & Precision Pathology Center ( BRCP ) as well as the Research Immunohistology Lab. Full blood and key organs were preserved for clinical chemistry and histopathological studies in the Animal Histopathology and Laboratory Medicine Core at the UNC School of Medicine. Female, 10-week-old, and 12-month-old BALB/cAnNHsD mice were ordered from Harlan Laboratories. Animal histopathology was performed within the LCCC Animal Histopathology Core Facility at UNC, supported in part by NCI grant No. The UNC Animal Histopathology & Laboratory Medicine Core is supported in part by an NCI Center Core Support Grant (5P30CA016086-41) to the UNC Lineberger Comprehensive Cancer Center. The UNC Flow Cytometry Core Facility is also supported in part by the N.C. Biotechnology Center. Abnormal gut-associated lymphoid tissue (GALT) in humans is associated with infectious and autoimmune diseases, which cause dysfunction of the gastrointestinal (GI) tract immune system. Consultation is available for assistance with or … We hypothesized that this dichotomy is due to the inadvertent absence of caspase-11 in previously used caspase-1-deficient mice. Overview. Gut microbiota is thought to influence the immune response to oral vaccines. Contact Us University of Utah School of Medicine. Leist et al. We thank Ricardo Rivera-Soto for his help with the synergy experiments. Cancer Center Core Support. 9 Animal Histopathology Core, The University of North Carolina, Chapel Hill, NC 27599, USA. The Center is funded by NIH to support research in gastrointestinal biology at UNC-Chapel Hill and North Carolina State University. The formalin-preserved tissue was sliced with a scalpel blade and arranged in a cassette containing foam biopsy pads and submitted to the University of North Carolina (UNC) Animal Histopathology Core Facility for processing, embedding, sectioning, and H&E staining. The National Toxicology Program (NTP) Satellite Symposium is a 1-day meeting that has traditionally been held in conjunction with the annual Society of Toxicologic Pathology (STP) meeting (Adams et al. the University of North Carolina Animal Histopathology and Lab Medicine Core. Please contact her (stephanie_montgomery@med.unc.edu) if you have animal model needs with which she can assist. present a mouse model for COVID-19 by serially passaging human SARS-CoV-2 in vivo to create an evolution-selected lethal mouse-adapted virus variant, called MA10. Animal histopathology was performed by the Animal Histopathology & Lab Medicine Core at the University of North Carolina, which is supported in part by an NCI Center core Support Grant (5P30CA016086‐41) to the UNC Lineberger Comprehensive Cancer Center. Rotavirus vaccines have poor efficacy in infants from low- and middle-income countries. Animal histopathology was performed in the LCCC Animal Histopathology Core Facility at the University of North Carolina at Chapel Hill with special assistance from Traci Raley and Amanda Brown. Statistics Statistical analysis was performed using GraphPad Prism 7.03 software (La Jolla, CA). This work was supported by the NIA (AG024379 and AG036817), the NIEHS (T32 ES07126), and an HHMI training grant (Med into Grad Initiative). Study of ecosystem structure and energy flow. Pathology is the study of the causes and effects of disease or injury.The word pathology also refers to the study of disease in general, incorporating a wide range of biology research fields and medical practices. identify a key player that is critical for regulating CH methylation: the microRNA miR-29. The paraffin‐embedded clot slides were made and H&E staining was performed in the Animal Histopathology and Laboratory Medicine Core at UNC. Marie Brady 2800 Plymouth Rd, Bldg. Professor, Biological and Biomedical Sciences. All animal procedures were performed in accordance with the Guide for the Care and Use of Laboratory Animals (National Institutes of Health Publication 85-23, 1996) and approved by the Northwestern University Animal Care and Use Committee and the University of North Carolina Animal Care and Use Committee. Dr. Chen is a cancer researcher and has been funded continuously by NIH since 2002 to study molecular mechanisms, prevention, and targeted therapy of oro-esophageal cancer. The Institutional Animal Care and Use Committee (IACUC) of The University of North Carolina at Chapel Hill (UNC, Permit Number A-3410-01) approved the animal study protocol (IACUC #13-033) used in these studies. histopathology was done by UNC-CH Lineberger Comprehensive Cancer Center Animal Histopathology Core Lab Histology lab under the direction of Dr. Stephanie Montgomery. UVA Health’s Epidemiology and Emergency Management teams have together developed a response plan. Background To study spatiotemporal in vivo changes in retinal morphology and quantify thickness of retinal layers in a mouse model of light-induced retinal degeneration using spectral domain optical coherence tomography (SD-OCT). Blinded slides were assessed for virus associated lung pathology as well as location and prevalence of viral antigen. The Animal Histopathology Core is also currently in the process of expanding available animal immunohistochemical and immunofluorescent animal tissue labeling. ACVP, stephanie_montgomery@med.unc.edu Dawud Hilliard, MPM, HTL (ASCP) QIHC, dawud_hilliard@med.unc.edu. service grant (CA019014) to DPD. Formalin-fixed, paraffin-embedded tissue blocks from routine diagnostic surgical resections were transferred to UNC-CH under an approved Institutional Review Board protocol of the Cleveland Clinic. Female, 10-week-old, and 12-month-old BALB/cAnNHsD mice were ordered from Harlan Laboratories. The authors would like to thank the University of North Carolina Animal Histopathology and Flow Cytometry cores. UNC Bioinformatics Joel Parker, PhD Sara Selitsky, PhD UNC Core Facilities Translational Pathology Laboratory Genomic Pathology Laboratory Animal Histopathology Core Facility Weill-Cornell Kristy Richards, MD, PhD Acknowledgements 50. 8Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, UNC-Chapel Hill, NC 27599, USA 9Animal Histopathology Core, The University of North Carolina, Chapel Hill, NC 27599, USA 10Department of Pathology and Laboratory Medicine, The University of North Carolina, Chapel Hill, NC 27599, USA 11Department of Medicine, Division of Hematology and Oncology, The University of North ACVP, stephanie_montgomery@med.unc.edu Dawud Hilliard, MPM, HTL (ASCP) QIHC, dawud_hilliard@med.unc.edu. Animal Study Core. The Institutional Animal Care and Use Committee (IACUC) of The University of North Carolina at Chapel Hill (UNC, Permit Number A-3410-01) approved the animal study protocol (IACUC #13-033) used in these studies. I would like to thank the wonderful core facilities at University of North Carolina including Animal Studies Core especially Charlene Santos, Mark Ross and Alain Valdivia, Biomedical Research Imaging Center especially Dr. Hong Yuan, Animal Histopathology Core, Microscopy Services Laboratory and Animal Clinical Chemistry Core for their skills Histopathology analysis was performed in the UNC Animal Histopathology Core Facility, which is supported in part by an NCI Center Core Support Grant (2P30CA016086-40) to the UNC Lineberger Comprehensive Cancer Center. This work was supported in part by the National Cancer Institute Center Core Support Grant CA16086 and 5P30CA016086 to the UNC Lineberger Comprehensive Cancer Center for the UNC Lineberger Animal Studies Core and the UNC Animal Histopathology & Lab Medicine Core, respectively. Flow Cytometry and Cell Sorting Lungs were digested to single-cell suspensions by intratracheal instillation ORIGINAL RESEARCH 336 American Journal of Respiratory Cell and Molecular Biology Volume 60 Number 3 |March 2019 Positive mutant clones were used to produce chimeric animals by microinjection into C57BL/6J blastocysts. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Mortality differences were analyzed by the log-rank test. Precision Animal Models and Organoids Core Pilot & Feasibility Program This program hopes to encourage young investigators and more established investigators in other field to approach problems which are relevant to our understanding of normal intestinal, liver and pancreatic function and to … the UNC Animal Histopathology & Lab Medicine Core. ). We decided to develop a dual ISH-IHC protocol due to direct requests from the institute’s scientists, but also in part because of scientific curiosity that drive the innovation. Department of Genetics, University of North Carolina, 120 … The vivarium operates as a modified barrier facility and is equipped with quarantine and a procedure room, holding rooms with biosafety cabinets, an imaging/holding room equipped with a PerkinElmer IVIS SpectrumCT imaging system, and additional support areas. The Molecular Biology Histology facility maintains automatic tissue processor, tissue embedding center, rotary microtome, cryostat, and supplemental equipment. Mice and in vivo infection. The UNC Flow Cytometry Core Facility is supported in part by P30 CA016086 Cancer Center Core Support Grant to the UNC Lineberger Comprehensive Cancer Center. The UNC Flow Cytometry Core Facility is also supported in part by the N.C. Biotechnology Center. part of my role in the core facility. The image shows a hematoxylin and Eosin (H&E) stain of a liver section belonging to an NSG mouse with metastatic infiltrate. Cutting-edge Diagnostic Testing Services . Stephanie A. Montgomery, PhD, DVM, Dipl. Serum TNF-α and INF-γ levels were quantified using multiplexed biomarker immunoassays performed by the Animal Histopathology and Lab Medicine Core at the UNC School of Medicine. Department of Microbiology and Immunology School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. The use of inbred mice housed under standardized environmental conditions has been critical in identifying immuno-pathological mechanisms in different infectious and inflammatory diseases as well as revealing new therapeutic targets for clinical trials. The Institutional Animal Care and Use Committee Guidelines for Use of Animal Core Facilities Introduction The Institutional Animal Care and Use Committee (IACUC) has developed these guidelines for researchers at the University of North Carolina at Chapel Hill who provide or receive Core … 35 Ann Arbor, MI 48109-2800 ph. The UNC Flow Cytometry Core Facility, the UNC Translational Pathology Laboratory, the Animal Histopathology and Laboratory Medicine Core, and the UNC Lineberger Animal Studies Core are supported in part by P30 CA016086 Cancer Center Core Support Grant to the UNC Lineberger Comprehensive Cancer Center. McGovern Medical School is the sixth largest medical school in the United States. S. H. Randell was supported in part by NIH 5P30DK065988. Olulanu H. has 6 jobs listed on their profile. The UNC Flow Cytometry Core Facility is also supported in part by the N.C. Biotechnology Center. I started using RNAscope® ISH just over a year ago and haven’t looked back. The Animal Studies Core, affiliated with the UNC Lineberger Comprehensive Cancer Center, provides full access to a range of cancer cell lines and animal models, including genetically modified mouse models of human cancers. We acknowledge UNC Animal Histopathology Core and UNC Translational Pathology Laboratory for tissue embedding, IF, and IHC staining. Nanoparticles (NPs). In the journal Cell Reports, UNC Lineberger researchers reported they found markedly low levels of the protein NLRX1 in multiple laboratory models of colorectal cancer, and in samples of human tissue. NC State Veterinary Medicine diagnostic laboratories serve veterinarians, scientists, educators, companion animal owners, and the livestock and poultry industry by identifying and monitoring emerging diseases, genetic disorders and …

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